Methods: Our study initiated from a cohort of 1,619 IIM patients (BIRD/University of Bath serology service and UKMyoNet cohorts) and resulted in 157 unique serum samples enriched for higher prevalence of MSA characterized by the laboratory's routine methods, IP and line immunoassay (LIA: Euroimmun). Here we aimed to compare three methods for the detection of MSA. Therefore, reliable alternatives to IP are mandatory. Many laboratories rely on immunoprecipitation (IP) for the detection of MSA but this approach is compromised by logistic, standardization, and regulatory challenges. Objectives: Standardization of myositis specific antibody (MSA) detection is of high importance because these antibodies are relevant for diagnosis and stratification of patients with idiopathic inflammatory myositis (IIM) and have the potential to be used in classification criteria. 4NIHR Manchester Biomedical Research Centre, Manchester University NHS Foundation Trust, The University of Manchester, Manchester, United Kingdom.3Rheumatology Department, Salford Royal NHS Foundation Trust, Manchester Academic Health Science Centre, Salford, United Kingdom.2Department of Pharmacy and Pharmacology, University of Bath, Bath, United Kingdom.1Inova Diagnostics, Inc., San Diego, CA, United States.Michael Mahler 1 * Zoe Betteridge 2 Chelsea Bentow 1 Michaelin Richards 1 Andrea Seaman 1 Hector Chinoy 3,4 Neil McHugh 2 on behalf of UKMYONET

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